You can use this website to download freeware applications (see links below) written by G. Mashanov (The Crick Institute, UK). This software was developed for the detection/tracking, analysis, and modelling of single molecule dynamics (movement and binding kinetics) in live cells, but it can be used for other purposes. You can download our real and simulated data samples and ImageJ Plugins to import and export your data files.
The software was compiled using CBuilder_XE7. It will run under Win32 or Win64 OS and does not require installation or registration:
1. Download required .zip file (see below). The archives contain ".exe" files (32 or 64 bit), and corresponding “.dll” files (“.bpl” libraries).
2. Unzip these files into selected folder on your computer.
3. Run the required “.exe” file.
You may manually associate “.gmv” data files (using Windows Explorer) with GMimPro and “.gmi” files with Motility to open data files by clicking on it. I am happy to answer your specific questions firstname.lastname@example.org but, please, read the help files (.pdf) (and corresponding publications) first.
GMcellModel is a computer model simulating mobility and binding kinetics of the single fluorescent molecules (both cytoplasm and membrane associated) in a virtual cell. It generates a sequence of images (8-bit “.bmp” or “.gmv” format), each containing summed images of all fluorescent objects emitting light under given illumination conditions with realistic levels of noise and emission fluctuations. These sequences can be analysed by GMimPro or other imaging software (e.g., ImageJ). You can load few basic scenarios (downloaded folder GMcellModelScenario) and run the model to test it. See JRS Interface 2014 publication for full description of the employed algorithms.
Motility is a satellite software designed for statistical analysis of tracking data (.gmi) generated by GMimPro. You can add many individual “.gmi” files together and create distributions of: intensity, mobility, velocity, and other paprameters. You can create plots of average intensity, mobility, and "distance from the origin" versus time, generate MSD versus dT plots, and others. You can apply thresholds to separate slow-fast, dim-bright, short-long lived objects, and so on. The graphs can be printed, saved as “.bmp”, and exported as “.txt” files for future analysis or publishing.
ImageJ plug-ins are written by Dr. J.E. Molloy (The Crick Institute, UK).
1. Copy plugins into ImageJ plugins folder
2. Open ImageJ and load your image sequence
3. Input scales and time interval to the sequence properties if needed
4. Use“GMV Writer” in “Plugins” menu to save your data as “.gmv” file.
Alternatively you can save your data as RAW data file and use File/Import Data in GMimPro to convert data into GMimPro format (".gmv").
Download 64bit “.exe” files and libraries
Download ImageJ Plugins for GMimPro
Download Data samples
GFP_inVitro - single GFP molecules attached to glass via antiGFP ab (in vitro, TIRF microscopy)
Cy3B_inVitro - single fluorescent molecules of Cy3B dye attached to coverslip (in vitro, TIRF microscopy)
GFP_A1_HEK - Adenosine GPCR A1 receptors (GFP tagged) at plasma membrane of live HEK cell (37°C, TIRF microscopy)
Cy3B-Tz_M1_CHO - Muscarinic Acetylcholine GPCR M1 receptors at plasma membrane of live CHO cell (23°C, labelled with Cy3B-telenzepine, TIRF microscopy)
Cy3B-Tz_M2_CHO - Muscarinic Acetylcholine M2 receptors at plasma membrane of live CHO cell (23°C, labelled with Cy3B-telenzepine, TIRF microscopy)
Cy3B-Tz_M2_HL1 - Muscarinic Acetylcholine GPCR M2 receptors at plasma membrane of live HL1 cell @23˚C (37°C, labelled with Cy3B-telenzepine, TIRF microscopy)
Cy3B-Tz_M2_HeartSlice - Muscarinic Acetylcholine GPCR M2 receptors at plasma membrane of ex-vivo mice heart slice (23°C, labelled with Cy3B-telenzepine, TIRF microscopy)
GFP_KCNQ1_HEK - KCNQ1 potassium channels. GFP tagged at plasma membrane of live HEK cell (37°C, TIRF microscopy)GFP_KIR6.2_HEK – KIR6.2 potassium channels. GFP tagged at plasma membrane of live HEK cell (37°C, TIRF microscopy)