My name is Gregory Mashanov. I work in the Francis Crick Institute (London, UK). My main areas of research are: single molecule imaging in live cells, modelling single molecule dynamics in live cells, writing software for scientific projects.
1962 Born in Ulan-Ude, Eastern Siberia, USSR.
1973 Moved to Chelyabinsk (Urals).
1979 Finished secondary school N106 in Chelyabinsk.
1979–1984 Full course of biology (Char of Physiology) at Urals State University, Sverdlovsk (Ekaterinburg).
1986-1995 Worked in Medical Research Institutes in Ekaterinburg
1996-1999 Institute of Physiology, Urals Branch of RAS, Ekaterinburg.
1997 PhD in Biophysics, Biology Department of Moscow State University.
1999-2002 Postdoc in J.E. Molloy lab, University of York, UK
2002-2015 National Institute for Medical Research, London, UK
2015- The Francis Crick Institute, London, UKPublications:
Baboolal T.G., Mashanov G.I., Nenasheva T.A., Peckham M., Molloy J.E., (2016) A combination of diffusion and active translocation localizes Myosin-10 to the filopodial tip, J. of Biol. Chem., 291:22373-22385.
Conte I.L., Hellen N., Bierings R., Mashanov G.I., Manneville J.B., Kiskin N.I., Hannah M.J., Molloy J.E., Carter T. (2015) Interaction between MyRIP and the actin cytoscleleton regulates Weibel-Palade body trafficing and exocytosis, J. of Cell Science, 129:592-603.
Conte I.L., Coolson E., Hellen N., Bierings R., Mashanov G.I., Carter T. (2015) Is there more than one way to unpack a Weibel-Palade body? Blood, 126:2165-2167.
Mashanov G.I. (2014) Single molecule dynamics in a virtual cell: a three-dimensional model that produces simulated fluorescence video-imaging data, JRS Interface, 11:1-11
Nenasheva T.A., Neary M., Mashanov G.I., Birdsall N.J.M., Breckenridge R.A. and J.E. Molloy (2013) Abundance, Distribution, Mobility and Oligomeric State of M2 Muscarinic Acetylcholine Receptors in Live Cardiac Muscle, J. of Molecular and Cellular Cardiology, 57:129-136.
Chisty L.T., Toseland Ch.P., Fili N., Mashanov G.I., Dillingham M,S.. Molloy J.E., Webb M.R. (2013) Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD, Nucleic Acids Research, 41:5010-5023
Nenasheva T.A., Carter T., Mashanov G.I. (2012) Automatic tracking of individual migrating cells using low-magnification dark-field microscopy. J. of Microscopy, 246:83–88.
Nenasheva T.A., Mashanov G.I., Peckham M, Molloy J.E. (2011) Imaging individual myosin molecules within living cells. Methods in Molecular Biology, 778:123-142.
Fili N., Mashanov G.I., Toseland Ch. P., Batters Ch., Wallace M.I., Yeeles J.T.P., Dillingham M.S., Webb M.R., Molloy J.E. (2010) Visualizing helicases unwinding DNA at the single molecule level, Nucleic Acids Research, 38:4448-4457
Hern J.A., Baig A.H., Mashanov G.I., Birdsall B., Corrie J.E.T., Lasareno S., Molloy J.E., Birdsall N.J.M. (2010) Formation and dissociation of M1 muscarinic receptor dimers seen by total reflection fluorescence imaging of single molecules, PNAS, 107:2693-2698.
Mashanov G.I., Nobles M., Harmer S.C., Molloy J.E., Tinker A. (2010) Direct Observation of Individual KCNQ1 Potassium Channels Reveals Their Distinctive Diffusive Behavior, J. of Biol. Chem., 285:3664-3675
Mashanov G.I., Molloy J.E. (2007) Automatic detection of single fluorophores in live cells. Biophys.J., 92:2199-2211.
Mashanov G.I., Nenasheva T.A., Peckham M, and J.E. Molloy. (2006) Cell biochemistry studied by single-molecule imaging, Biochemical Society Transactions, 34:983-988.
Nenasheva T.A., Mashanov G.I. (2006) Visualization of single fluorescent molecules in living cells (Review) Biofisika (Russia)., 51:454-465.
Mashanov, G.I., Tacon, D., Peckham, M., and J.E. Molloy. (2004) The spatial and temporal dynamics of pleckstrin homology domain binding at the plasma membrane measured by imaging single molecules in live mouse myoblasts. J. Biol. Chem., 279:15274-15280.
Mashanov, G.I., Tacon, D., Knight, A.E., Peckham, M., and J.E. Molloy. (2003) Visualizing single molecules inside living cells using total internal reflection fluorescence microscopy. Methods, Academ. Press., 29:142-152.
Mashanov G.I. (1997) The cross-bridge model for an in vitro motility assay. Biofisika (Russia), 42:1113-1121 (in Russian).
Bershitsky S.Y, Tsaturyan A.K, Bershitskaya O.N, Mashanov, G.I, Brown P, Burns R, Ferenczi M.A. (1997) Muscle force is generated by myosin heads stereospecifically attached to actin. Nature, 388:186-190.
Mashanov G.I. (1997) The cross-bridge model for an in vitro motility assay. J. of Muscle Research and Cell Mot. 18:265.
Mashanov G.I. (1997) The mechanical interaction of skeletal muscle actin and myosin in an in vitro motility assay. PhD Thesis, Moscow State University (150 pages)
Bershitsky S.Y, Tsaturyan A.K, Bershitskaya O.N, Mashanov G.I, Dobbie I, Irving M, Torok K, Brown P, Ferenczi M.A. (1996) The mechanical and structural properties of permeabilised skeletal muscle fibers of the frog after partial EDC cross-linking. J. of Muscle Research and Cell Mot., 17:156.
Bershitsky, S.Y, Tsaturyan A.K, Bershitskaya O.N, Mashanov G.I, Brown P, Webb M.R. and Ferenczi M.A. (1996) Mechanical and structural properties underlying contraction of skeletal muscle fibres after partial 1-ethyl-3[3-(dimethylamino) propyl] carbodiimide cross-linking. Biophys.J., 71:1462-1474.
Mashanov G.I, Tsyvian P.B, Artemeva O.G. (1992) Early hypoxic contracture in adult and new-born rat myocardium. Physiological Journal (Kiev, Ukraine), 38:36-41 (in Russian).
Mashanov G.I, Tsyvian P.B, Abazov V.A. (1990) Temperature controller for the small perfusion chambers. Physiological Journal of USSR (in Russian), 6:422-424.
Tsyvian P, Mashanov G.I, Artemeva O. (1989) Early hypoxic contracture in adult and new-born rat myocardium. J. of Molecular and Cellular Cardiology, 21:60.